IanN 0 Report post Posted September 4, 2018 Hi There appears to be an issue with some of the calculated X-Median values. The attached picture shows data displayed using legacy mode (on data acquired with a FACSCanto II). Looking at the B cells (blue histograms) the peaks are between 10 and 100 on the log scale so I would expect the X-Median values to be > 0.10. Given the T cells X-medians are 0.95 and they're in the lower decade, I struggling to work out how these values are arrived at. I presume the 20-bit bug issue has been addressed in this release. Is there something intrinsically different in the way the calculations are performed in this version? Quote Share this post Link to post Share on other sites
Ernie 0 Report post Posted September 5, 2018 Ian, Median is the "middle value". When you have smaller quantities of data that don't form a clear distribution, the median can be surprising. I sent you a forum message with my email address. If you'll send me a copy of the .analysis this comes from, I'd be happy to look into it. Ernie Quote Share this post Link to post Share on other sites
