Jump to content
Beckman Coulter User Forums

X-Median calculation query (v2.1)

Recommended Posts



There appears to be an issue with some of the calculated X-Median values. The attached picture shows data displayed using legacy mode (on data acquired with a FACSCanto II).


Looking at the B cells (blue histograms) the peaks are between 10 and 100 on the log scale so I would expect the X-Median values to be > 0.10. Given the T cells X-medians are 0.95 and they're in the lower decade, I struggling to work out how these values are arrived at. I presume the 20-bit bug issue has been addressed in this release. Is there something intrinsically different in the way the calculations are performed in this version?




Link to comment
Share on other sites



Median is the "middle value". When you have smaller quantities of data that don't form a clear distribution, the median can be surprising. I sent you a forum message with my email address. If you'll send me a copy of the .analysis this comes from, I'd be happy to look into it.



Link to comment
Share on other sites

Join the conversation

You can post now and register later. If you have an account, sign in now to post with your account.

Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.


  • Create New...