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Median calculation between single file and composite


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Hi,

 

I have a little problem with the median calculation in Kaluza 1.5a. If I open a fcs file the median values are very small (see picture 1) and the first decade of a Dot Plot is 100. After I create a composite the same values are multiplicate by the factor 1000 and the first decade is 103 (picture 2). That’s really a problem. I can’t compare the median values from single fcs files analyses with composite analyses. Is there any Workaround for this problem?

post-88352-0-82057900-1492597774_thumb.jpg

post-88352-0-61501500-1492597786_thumb.jpg

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  • 2 weeks later...

Your "Protocol Scale Mode" for one protocol is "Legacy" and the other is "Full Range". This is available from the context menu of the analysis list. See page 2-84 of the IFU.

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