ned Posted August 4, 2015 Report Share Posted August 4, 2015 Does anyone have any experience using a crystalography plate on a Biomek FX with a 96-well head? A scientist here asked if we have a robot capable of filling these plates: http://hamptonresearch.com/product_detail.aspx?sid=182&pid=82They're a 96-well format, but within each well area there is 1 reservoir and 2-3 smaller wells (smaller than the wells of a 384-well plate) which hold at most 1-2ul. They would be filled with a combination of solvent (stamped from a source plate using the 96-tip head) and protein (from a tube, using one or more Span-8) tips. Has anyone done this? I don't know what level of accuracy we need, but it doesn't need to be too accurate. Link to comment Share on other sites More sharing options...
Ulrich Vogel Posted November 5, 2015 Report Share Posted November 5, 2015 Some of these plates seem to be easy to treat as 384 well plates when pipetting at fixed heights and ignoring liquid volumes / filling levels in the wells.I could imagine to run the individual transfers with specific Techniques/Templates to avoid touching the bottom. Some others have a more irregular format, so I would suggest to define different pieces of labware (96 well format) and swapping them with Instrument Setup steps as needed.But I could also imagine to have only one labware definition and use different Pipetting Templates where you move to the desired XYZ-position. For both cases make sure, the Auto-Select option for the Techniques is switched off so that the user must pick the correct Technique/Template manually! Ulrich I'm sorry for the delay, I just found this post (and some others) in another folder of the forum. It wasn't activated yet and I never received any notification about it.So I moved it here as this seems to be the proper location. Link to comment Share on other sites More sharing options...
ned Posted November 5, 2015 Author Report Share Posted November 5, 2015 We decided to use similar plates that look more like a 384-well plate. They still have 1 large reservoir and three smaller ones, but in a square. We can fill the large reservoirs using the Biomek's 96-channel head (treating it as a 96-well plate) and then fill the small wells using another instrument (a LabCyte Echo that's ideal for hit-picking very small volumes). Link to comment Share on other sites More sharing options...
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