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jamesmccracken's Achievements


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  1. Hi Karen, You should still be able to pass QC on either of these nozzles, as Alignment QC is based on being able to align to the core stream and the width of the sheath stream won't effect that. I'd make sure you have really gotten all the air out, especially from the nozzle assembly. Vertical stream is important, as is beads at a good concentration to allow for a low pressure differential when measuring CVs. If you still can't get all parameters to pass, think about what you are sorting and if those parameters will even be needed. If your far-red channels fail, but you are doing a violet channel only sort, then no problem.
  2. In response to a question on the PUCL mailing list here is the latest version of CytoCalc. Have fun with it! JamesSpec - MoFlo & XDP - CytoCalc v3.2.xls
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